Fig. 6: CEP104 and TOGARAM1 are sufficient to impart slow MT growth. | Nature Structural & Molecular Biology

Fig. 6: CEP104 and TOGARAM1 are sufficient to impart slow MT growth.

From: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth

Fig. 6

a, Field of view (left; scale bar: 2 µm) and kymograph (right; scale bars: 2 µm and 60 s) illustrating MT dynamics with GFP–EB3, mCherry–TOGARAM1 and GFP–CEP104. b–e, Parameters of MT plus-end dynamics in the presence of EB3 in combination with indicated concentrations of CTM proteins (from kymographs shown in a and Fig. 1c,l,p). For pause and block duration (b) and growth rates (d), data were pooled from three independent experiments. Total number of growth events, pauses: EB3 alone, n = 938, 0; EB3 with CEP104, n = 213, 101; EB3 with TOGARAM1, n = 861, 0; EB3 with CEP104 and TOGARAM1, n = 373, 140. Bars represent the mean ± s.e.m. For dynamic state (c) and transition frequencies (e), bars represent the averaged means from three independent experiments. Error bars represent the s.e.m. f, Scheme showing interactions between CEP104, TOGARAM1 and tubulin.

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