Extended Data Fig. 1: Characterization of CTM proteins in vitro. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Characterization of CTM proteins in vitro.

From: A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth

Extended Data Fig. 1

a, Analysis of purified GFP-tagged CTM proteins by SDS-PAGE. Asterisks indicate the full-length protein bands. Protein concentrations were determined from a BSA standard. Assays were repeated independently at least two times. b, SEC-MALS analysis of purified GFP-tagged CTM proteins. The human sequence was used for all module members, except for TOGARAM1. Due to technical difficulties, the mouse sequence of TOGARAM1 was used, which is 84% identical to human TOGARAM1. c, Mass spectrometry analysis of purified GFP-tagged CTM proteins. d, Fields of view (top, scale bar 2 µm) and kymograph (bottom, scale bars 2 µm and 60 s) illustrating MT dynamics from GMPCPP-stabilized seed with HiLyte-488-tubulin and mCherry-CEP104. e, Kymographs illustrating mobility of DmKHC(1-421) on CEP104-blocked MT labelled with HiLyte-488-tubulin and mCherry-CEP104 (top) and DmKHC (bottom) proving that the blocked end of the MT is the plus end. Scale bars 2 µm and 60 s for both kymographs.

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