Fig. 4: Time course of fucosylation inhibition.
a, Experimental design. b, PCA of the normalized TMT glycopeptide intensities per treatment condition, time point and replicate. PC, principal component. c, Glycopeptide modulation over time. Glycopeptides significantly regulated for at least one time point were grouped in four clusters depending on their kinetics of regulation using neural gas clustering. d, Proportion of glycan classes present in the four clusters. e, Fucosylated peptides being significantly downregulated for at least one time point were grouped into three clusters using neural gas clustering. f, Glycoproteins having fucosylated glycoforms belonging to the three clusters. Here, the mean log2 fold changes of the fucosylated glycopeptides belonging to each cluster are displayed. FC, fold change. g,h, The fucosylated glycoforms of the C-type mannose receptor 2 (MRC2) and the neuronal cell adhesion molecule (NRCAM) exhibit different rates of downregulation.
