Fig. 7: Systematic characterization of glycoform biophysical properties. | Nature Structural & Molecular Biology

Fig. 7: Systematic characterization of glycoform biophysical properties.

From: Uncovering protein glycosylation dynamics and heterogeneity using deep quantitative glycoprofiling (DQGlyco)

Fig. 7

a, Solubility proteome profiling experimental workflow, enabling the proteome-wide characterization of the in vivo biophysical properties of glycoforms by assessing their solubility in a nondenaturing detergent (NP40) and in a SDS. b, Glycoform solubility compared to the solubility of the general protein population per glycan class (two-sided t-test). The horizontal lines represent the median. c–e, Solubility profiling of glycoforms of proteins encoded by Cdh13 (c), Thy1 (d) and Cd47 (e). Each dot represents a glycoform while the solid lines represent the solubility of the general protein population, based on nonmodified peptides.

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