Extended Data Fig. 2: Cryo-EM data processing and reconstruction of the Mnl1-Pdi1 complex. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Cryo-EM data processing and reconstruction of the Mnl1-Pdi1 complex.

From: Initiation of ERAD by the bifunctional complex of Mnl1/Htm1 mannosidase and protein disulfide isomerase

Extended Data Fig. 2

(a) Representative cryo-EM image. (b) Cryo-EM processing workflow (see also Table 1). The classes selected for further analysis are colored. The pink class resulted in a structure of the entire Mnl1-Pdi1 complex (Complete). The class shown in light blue lacks density for the CTD of Mnl1 (Incomplete) and was subjected to another round of 3D classification. Three of the resulting classes were refined (Incomplete #1–3). (c) 3D Fourier shell correlation (FSC) curves for the pink class. The FSC calculations used the mask shown on the side. (d) Local resolution map for the pink class in two different views with scale on the right. (e) Euler angle distribution of refined particles of the pink class shown in two different views. (f)-(h) As in (c)-(e), but for the Incomplete #1 map that has the highest resolution among the incomplete maps.

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