Fig. 2: Inhibitors stabilize a BRISC dimer.
From: Molecular glues that inhibit deubiquitylase activity and inflammatory signaling
a, Mass photometry histograms of purified BRISC in absence (DMSO, left) and presence (JMS-175-2, middle; FX-171-C, right) of inhibitors. Insets, corresponding negative-stain EM 2D classes of BRISC mixed with DMSO or inhibitors. b, Left, cryo-EM density map of a BRISC monomer with a BRISC model (PDB 6H3C) rigid-body fitted (dust cleaning size: 7.4, map threshold: 0.0907). Right, cryo-EM density map of a BRISC dimer with two BRISC models rigid-body fitted. Maps are outputs from nonuniform refinement in cryoSPARC.
