Fig. 4: Analysis of the BLUE compound-binding site.
From: Molecular glues that inhibit deubiquitylase activity and inflammatory signaling
a, Ball-and-stick model of FX-171-C and JMS-175-2 binding to BRCC36, Abraxas 2 and BRCC45. Hydrogen bonds are shown as black dashed lines and residues studied by mutagenesis are indicated. b, The BLUE compound-binding pocket shown as a surface and colored by hydrophobicity. c, FX-171-C inhibition of BRISC DUB activity with BRCC36 (left), Abraxas 2 (middle) and BRCC45 (right) mutants. d, SHMT2 inhibition of the same BRISC mutants as in c. Data in c,d are the mean ± s.e.m. of three independent experiments carried out in technical duplicate.
