Extended Data Fig. 7: CryoEM data processing.
From: Shigella flexneri evades LPS ubiquitylation through IpaH1.4-mediated degradation of RNF213
(a) A representative micrograph, acquired at 1.5 μm defocus, from the IpaH1.4–RNF213 dataset (comprising 16,931 micrographs in total), low-pass filtered to 10 Å for display. (b) Representative 2D class averages of the IpaH1.4–RNF213 complex. (c) The flow chart summarizes the three-dimensional processing strategy, aimed at removing RNF213 particles without IpaH1.4 bound and at improving the resolution around the flexible region of the IpaH1.4 LRR and RNF213 RING. All processing steps after the initial consensus 3D refinement were performed in RELION. (d) Orientation distribution of the particles used in the final reconstruction of the IpaH1.4–RNF213 complex, plotted on a Mollweide projection. The efficiency of the orientation distribution, E, is 0.75. (e) Fourier shell correlation as function of resolution is plotted for the best consensus masked map (green), the best masked map for the locally refined region of the IpaH1.4 LRR and RNF213 RING (orange), the unmasked consensus map (dark grey), and the phase-randomized consensus map (light grey).
