Extended Data Fig. 10: Nucleosome binding titrations indicate that Chd1[GSD/brace II]864-871-flex binds more tightly to the TA-rich side of the nucleosome.
From: A competitive regulatory mechanism of the Chd1 remodeler is integral to distorting nucleosomal DNA
This graph shows fits to a two-Kd model, where the Chd1[GSD/brace II]864-871-flex variant was titrated against 40N40 nucleosomes made with either canonical Widom 601 or the 601[TArich+1] sequence. For these experiments, the higher affinity site becomes stronger with 601[TArich+1] nucleosomes, suggesting that the higher affinity site for Chd1[GSD/brace II]864-871-flex is the TA-rich side. Data are presented as mean values +/− SD from n independent replicates. Data are given in Source Data File 10.
