Extended Data Fig. 4: Generation of knockout and rescue cell lines.
From: Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
a, Cell lysates from WT and ATG13 KO HeLa cells were analyzed by immunoblotting with indicated antibodies. b, Cell lysates from ATG13 KO cells expressing Halo-LC3B without rescue or rescued with indicated versions of HA-ATG13 were immunoblotted with anti-ATG13 and anti-Actin antibodies. These experiments were not repeated because they were designed to confirm the expression of proteins.
