Extended Data Fig. 5: Structural determination of the ULK1C (2:2:2) core in the PI3KC3-C1 mixture and the ATG13450-517truncation mutant.
From: Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
a, A representative cryo-EM micrograph of the ULK1C:PI3KC3-C1 mixture sample. b, Representative 2D class averages of four subpopulations of the dataset. From left: PI3KC3-C1 alone, ULK1C:PI3KC3-C1 supercomplex, junk particles, and ULK1C alone. c, Result of the homogeneous refinement of the ULK1C alone substack. The FSC, local resolution, and angular distribution are shown accordingly. The EM map is contoured at 7σ. d, Size exclusion chromatography (SEC) profile of the ULK1C (2:2:2) core of ATG13450-517 truncation mutant. The inset shows an SDS-PAGE of the peak (red bar). e, A representative cryo-EM micrograph of the ULK1C core containing the ATG13450-517 truncation mutant. f, Representative 2D class averages. g, Result of the first round of 3D classification. h, Global refinement of the final substack. The FSC, local resolution, and angular distribution are shown accordingly.
