Fig. 4: Structure of the ULK1C:PI3KC3-C1 supercomplex.
From: Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
a, Representative 2D class average of the ULK1C:PI3KC3-C1 supercomplex. Scale bar, 20 nm. b, Overview of the EM map and the coordinates of ULK1C:PI3KC3-C1 supercomplex superposed with the EM map contoured at 8σ. Note: only one FIP200 molecule of the ULK1C was modeled (blue). c, Close-up view of the FIP200-binding interface of PI3KC3-C1. FIP200 is shown in surface representation and PI3KC3-C1 is shown in ribbon representation. The FIP200ULD, VPS15HSD, BECN1, CC and zinc-finger domains formed by BECN1–ATG14 are indicated. d, Close-up view of the unassigned EM densities at sites 1 and 2. The local refinement map is shown at 22σ. The assigned map is colored according to the molecule (blue, FIP200; pink, VPS15; green, BECN1; yellow, ATG14). The extra densities observed at site 1 are shown in gray and indicated by arrows.
