Extended Data Fig. 3: Cryo-EM data processing workflow for the dynein + Lis1 + ATP 30 min dataset.
From: Multiple steps of dynein activation by Lis1 visualized by cryo-EM
Dose-weighted movies from a dataset of dynein with Lis1 (30 min condition) were aligned with MotionCor2 and CTF was estimated using CCTFFIND4. Particle extraction (binned by 4) was performed in cryoSPARC with a Topaz-trained model. Good particles from 2D classification jobs were used for ab-inito model generation in cryoSPARC. The models in red boxes were carried into the next steps. Fourier shell correlation (FSC) plots are shown next to the final maps.
