Extended Data Fig. 10: Comparison to prior structures shows broad conservation of TRIP12-donor Ub arrangement.
From: TRIP12 structures reveal HECT E3 formation of K29 linkages and branched ubiquitin chains
a, Superimposition of HECT C-lobe and donor Ub (UbD) from structure of TRIP12 with structures of UBR5 (PDB 8C07), Rsp5 (PDB 4LCD), HUWE1 (PDB 6XZ1, 6FYH), NEDD4 (PDB 4BBN), NEDD4L (PDB 3JW0) and SMURF2 (PDB 6FX4) (left), or with Ufd4 (PDB 8J1P, right). Structures were aligned over their C-lobes. b, Comparison of positioning of the acceptor Ub (UbA) in the HECT C-lobe interface between structures for TRIP12 (left, acceptor Ub in orange) and Ufd4 (center, acceptor Ub in red), or both superimposed (right). Structures were aligned over their C-lobes.
