Extended Data Fig. 2: Characterization of H2A.Z loss in FGOs.
a, Volcano plots showing differentially modified regions of H2A.Z at two adjacent stages. H2A.Z-lost peaks are highlighted (FDR < 0.05, |FC | > 2). FDR is calculated by Benjamini-Hochberg adjusted p-value (two-sided) of glmLRT in ‘edgeR’. b, Genomic distribution of H2A.Z peaks that are lost or retained in FGOs compared with 15 d GOs. c, Transcription factor motifs identified at H2A.Z-lost promoters (left). Right bubble plots represent the expression level of TFs in FGOs and the statistical significance of the motif enrichment (P-value of hypergeometric test with one-sided). d, Box plots showing expression levels of the genes whose promoters lose or retain H2A.Z in FGOs. The center lines in the boxes represent median values. The box edges, upper and lower whiskers indicate the interquartile range (IQR, from the 25th to 75th percentile), the maximal value smaller than 1.5 x the IQR above the 75th percentile, and the minimal value larger than 1.5 x the IQR below the 25th percentile, respectively (n = 1 as biological replicates were combined). The number of H2A.Z-lost or H2A.Z-retained genes are 1,799 and 10,567, respectively. e, Heatmaps showing the H2A.Z enrichment at bivalent promoters in DMSO-, DRB- and α-amanitin-treated GOs. f, Genome browser views showing the loss of H2A.Z at bivalent promoters in DRB- and α-amanitin-treated GOs.
