Extended Data Fig. 6: Sequence conservation strongly influences the effect of variation on FIX secretion.
From: Multiplex and multimodal mapping of variant effects in secreted proteins via MultiSTEP
a, Comparison of light chain secretion scores with Consurf conservation grades (1: least conserved, 9: most conserved)34. Violin plot shows distribution of points (n = 8,528 variants) with an inset box plot representing the 25th, 50th, and 75th percentiles. Whiskers span the range of data. Dashed horizontal line is the 5th percentile of the synonymous secretion score distribution. b, Comparison of median light chain secretion scores (n = 8,528 variants) with Consurf conservation grades. Violin plot shows distribution of points with an inset box plot representing the 25th, 50th, and 75th percentiles. Whiskers span the range of data. Dashed horizontal line is the 5th percentile of the synonymous secretion score distribution.
