Extended Data Fig. 10: Detection of cell-surface displayed FVIII.
From: Multiplex and multimodal mapping of variant effects in secreted proteins via MultiSTEP
Experimental flow cytometry of B-domain deleted coagulation factor VIII (FVIII) in the MultiSTEP backbone (n = ~30,000 cells per variant). Unrecombined cells (NC) do not display FVIII and serve as a negative control. Fluorescent signal was generated by staining cells with anti-FVIII antibodies specific to each of the five FVIII domains in the heavy chain [A1 (a) and A2 (b)] and light chain [A3 (c), C1 (d), and C2 (e)].
