Extended Data Fig. 3: MultiSTEP-derived FIX secretion scores correlate with orthologous measures of FIX secretion.
From: Multiplex and multimodal mapping of variant effects in secreted proteins via MultiSTEP
a, Flow cytometry of p.C28Y and WT controls (n = 10,000 cells) with the FIX library (n = 100,000 cells). b, Comparison of ELISA measurements of eight untethered FIX missense variants (p.C28Y, p.A37T, p.G58E, p.E67K, p.G125V, p.C134R, p.S220T, and p.H267L) expressed from 293-F cells and heavy chain secretion scores (n = 3 replicates). Error bars show the standard error of the mean. Pearson’s correlation coefficient is shown. c, Scatter plot comparing MultiSTEP-derived heavy chain secretion scores for 20 different FIX missense variants, WT, and unrecombined negative control (n = 3 replicates) to the geometric mean of Alexa Fluor-647 fluorescence measured using flow cytometry on cells expressing each variant individually. Error bars show standard error of the mean (n = 10,000 cells). Line of best fit (dashed) and Pearson’s correlation coefficient are shown.
