Extended Data Fig. 5: Impact of RNA-coordinating resides on yeast cell growth.
From: Structure of fungal tRNA ligase Trl1 with RNA reveals conserved substrate-binding principles
a, Yeast growth assay of the indicated strains to test cell survival of controls and after introducing Trl1 mutations. Dilution series (5-fold) of yeast cells were spotted on the indicated selective solid media. b, Overview of RNA binding amino acids in CtTrl1 (based on PDB ID: 8RBJ) as well as the corresponding residues in yeast (ScTrl1). The cell viability of the respective alanine variants in S. cerevisiae is indicated based on this study or in42.
