Table 1 Crystallographic data collection and refinement
From: Structure of fungal tRNA ligase Trl1 with RNA reveals conserved substrate-binding principles
CtTrl1-LIG–RNA | |
|---|---|
PDB ID | |
Data collection | |
Space group | P212121 |
Resolution (Å) | 2.37–47.6 (2.37–2.46) |
Unique reflection | 20384 (2019) |
Cell dimensions | |
a, b, c (Å) | 51.56, 75.36, 124.56 |
α, β, γ (°) | 90, 90, 90 |
Rmerge | 0.355 (3.123) |
Rpim | 0.090 (0.856) |
Mean (I/σ(I)) | 7.7 (1.0) |
Multiplicity | 15.8 (13.6) |
Completeness (%) | 100 (100) |
CC1/2 | 0.989 (0.392) |
Refinement | |
Rwork (%) | 17.87 (29.59) |
Rfree (%) | 21.78 (37.04) |
r.m.s.d. bond length (Å) | 0.007 |
r.m.s.d. bond angle (°) | 0.950 |
Ramachandran favored (%) | 97.4 |
Ramachandran allowed (%) | 2.3 |
Ramachandran outliers (%) | 0.3 |
Rotamer outliers (%) | 1.2 |
Clash score | 2.5 |
Average B factor (Å2) | 60.3 |
Protein | 60.4 |
Ligands | 48.2 |
Solvent | 47.7 |
