Extended Data Fig. 5: RNA is required for snRNA complex formation.
From: Structural basis for the synergistic assembly of the snRNA export complex
a. Overlay of Superdex 200 gel filtration elution profiles of the snRNA complex reconstitutions with different lengths of RNA. b. SDS-PAGE analysis of fractions 1–12 of the Superdex 200 gel filtration elution profiles shown in a. c. Mass photometry analysis of the snRNA complex formed with phosphorylated FL PHAX in the presence or absence of 14nt capped RNA. The measured molecular mass the full complex and the percentage of corresponding observations is indicated. d. Overlay of Superdex 200 gel filtration elution profiles of the snRNA complex reconstitutions with using either WT or the R147E, Y152E, Y154E mutant of MBP-PHAX103–196 and a short capped 14nt RNA. The WT complex elutes in fractions highlighted in red. e. SDS-PAGE analysis of fractions 1–12 of the gel filtration elution profile shown in c. MBP-PHAX103–196 R147E, Y152E, Y154E is unable to form the complex with CBC and CRM1-RanGTP. The fractions corresponding to the WT complex elution peak are highlighted with red rectangle.
