Extended Data Fig. 3: CBC Binding of ALYREF is incompatible with binding of PHAX and CRM1.
From: Structural basis for the synergistic assembly of the snRNA export complex
a-c. Comparison of CBC interactions with PHAX and CRM1 (a) with the CBC contacts with ALYREF (b) (PDB:8SRR, shown in magenta). The two structures were superimposed using CBC (c). The binding of ALYREF to the CBC is incompatible with that of PHAX and CRM1. (d) Western blot analysis of lysates from WT or HA-dTAG-PHAX mES cells harvested at indicated time points (hours) following dTAGV-1 treatment. Membranes were probed with antibodies against PHAX, HA, CRM1, Ran, ARS2 and CBP80. ACTIN was used as a loading control. (e) Western blot analysis of lysates from WT control or HA-dTAG-PHAX mES cells expressing MYC-miniTurbo(mT)-PHAX proteins. Cells were treated with dTAGV-1 to deplete endogenous dTAG-PHAX and biotin was added for 6 h to induce proximity labeling. Membranes were probed with MYC and ACTIN to assess protein expression and Streptavidin-HRP to assess biotinylation efficiency of mTurbo-tagged proteins.
