Extended Data Fig. 5: Glycerol fails to restore the Imh1 localization in glycerol channel mutant cells.
From: Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1
(a) Expression of Stl1 and Fps1 could rescue the localization of Imh1 in stl1∆, and fps1∆ cells, respectively. Cells expressing with mCH-Imh1 was coexpressed with Stl1 or Fps1 in stl1∆ or fps1∆ cells, respectively. Live cells were observed in mid-log phase. (Scale bar, 5 μm.) Model depicts glycerol entry in yeast cells. (b) Glycerol fails to restore the localization of Imh1 in stl1∆ and fps1∆ cells. mCH-Imh1 was expressed in gpd1∆, fps1∆, stl1∆, and gpd1stl1∆ cells. Cells were grown to mid-log phase in YPD, then medium was removed and cells were suspended in YP containing 0.1 M glycerol for 10 min. (Scale bar, 5 μm.) (a-b) The number of mCH-Imh1 puncta was determined using ImageJ Fiji software (N = 3, n = 50). Data are presented as the mean ± SD of three independent experiments. (N.S., not significant; two-sided unpaired t test).
