Extended Data Fig. 7: Intrinsic tryptophan fluorescence of Imh1-C213 is affected in the presence of glycerol.
From: Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1
(a) AlphaFold3 prediction model69 of His-Imh1-C213 dimer. The tryptophan residue in the 59th residue (magenta W59) is shown as stick model. (b) Intrinsic tryptophan fluorescence emission spectra of Imh1-C213 after treatment with 0.1 or 0.2 M glycerol. Similar results are shown in Supplementary Fig. 1. The sample was excited at 280 nm and the emission spectra from 300 nm to 450 nm were recorded as described in Methods.
