Extended Data Fig. 8: Differential cross-linked peptide pairs on Imh1-C213 identified in the absence and presence of 0.1 M glycerol.

(a) Six differently cross-linked peptide pairs on Imh1-C213 were identified, as highlighted in bold in Supplementary Table 1, both in the absence and presence of 0.1 M glycerol. The identified cross-linked peptide pairs are highlighted in squares, with the residues numbered according to their positions in full-length Imh1. The color and number of each square correspond to the specific cross-linked peptide pairs associated with the six sets of spectra presented in panel b. (b) Mass spectra (MS1) and tandem mass spectra (MS2) of the six differential cross-linked peptide pairs on Imh1-C213, regulated by glycerol, are presented. The upper panel displays extracted ion chromatograms generated using Xcalibur 2.2 software (Thermo Fisher Scientific). The matches for b and y ions of the cross-linked peptides, shown in the lower panel, were annotated using pLink3 and visualized with pLabel software (version 2.4.3), as detailed in the Methods section.