Extended Data Fig. 9: Glycerol rescues Imh1 and Snc1 localization in gpd1Δ cells under ER stress.
From: Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1
Cells expressing mCH-Imh1 and GFP-Snc1 were cultured to mid-log phase in YPD medium, and then incubated with or without 0.1 M glycerol, followed by treatment with or without 1 µg/ml TM for 1.5 h. Cells showing GFP-Snc1 plasma membrane signal were quantified (N = 3, n = 50). The number of mCH-Imh1 puncta was determined using ImageJ Fiji software (N = 3, n = 50). Data are presented as mean ± SD of three independent experiments. (N.S. Not significant; one-way ANOVA with Dunnett’s post hoc multiple comparison test, no glycerol addition was used as reference). Scale bar, 5 μm.
