Fig. 1: Single-particle Pf80S ribosomes.

a–c, Single-particle cryo-EM reconstructions, at 2.4–2.8-Å resolution, of the Pf80S ribosome in the POST state with P-site and E-site occupied, with the L1 stalk indicated (a), non-rotated PRE state with P-site, E-site and A-site occupied (b) and rotated PRE state with P-site and E-site occupied (c). The map is colored as follows: 60S large subunit, light gray; 40S small subunit, dark gray; A-site, P-site and E-site tRNAs, green, cyan and yellow, respectively. LSU, large subunit; SSU, small subunit.