Fig. 3: Ensemble of in situ Pf80S ribosomes comprising the malarial translation elongation cycle. | Nature Structural & Molecular Biology

Fig. 3: Ensemble of in situ Pf80S ribosomes comprising the malarial translation elongation cycle.

From: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Fig. 3

a–i, Native structures of the P. falciparum translational machinery, determined by in situ cryo-ET, elucidate the molecular ensemble of Pf80S ribosomes in malaria parasites. Directions of the small subunit rotation, backroll and head swivel are indicated on a cartoon of an 80S ribosome relative to the large subunit. Translation intermediate states (a–h) and unloaded ribosomal states (i) distinguished by position of principal ligands in these high-resolution STA reconstructions, enabling reconstitution of the P. falciparum translation elongation cycle. The refined map for each state is reconstructed from all particles corresponding to a particular class across datasets. Maps are shown in cross-section for clarity. eEF1α, dark red; A-tRNA, green; L1 stalk movement, pink; A/P-tRNA, teal; P-tRNA, cyan; eEF2, salmon; E-tRNA, orange.

Back to article page