Extended Data Fig. 5: Details of subtomogram averaged maps and perturbations of the translational landscape under CBQ-treatment. | Nature Structural & Molecular Biology

Extended Data Fig. 5: Details of subtomogram averaged maps and perturbations of the translational landscape under CBQ-treatment.

From: Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug

Extended Data Fig. 5

a, Examples of nascent chain density visible in the exit tunnel of POST, decoding-1 and decoding-2 and unloaded states. b, Bar graphs showing the translational landscape comprised of relative distribution of identified translation intermediates and ribosomal states in CBQ-treated and DMSO-control parasites. Percentages are based on total amount of identified ribosomes after particle curation. c-d, Bar graph showing the percentage of elongation factor-bound states across 4 time points in CBQ-treated and DMSO- control parasites, across four technical replicates. Percentages are based on total amount of identified ribosomes after particle curation (n = 230,471). Error bars represent SD from the mean. e, Relative soluble protein abundance of four CBQ-stabilized tRNA synthetases identified in the DIA Thermal PISA experiment: Arginine tRNA Ligase (ArgRS), Alanine tRNA Ligase (AlaRS), Leucine tRNA Ligase (LeuRS), Tryptophan tRNA Ligase (TrpRS). Protein abundance levels under CBQ treatment are plotted relative to the DMSO vehicle in each respective thermal gradient (n = 4 biological replicates). Significance changes (moderated t-test) are represented by asterisks (p value < 0.01 ‘**’; < 0.001 ‘***’). Error bars represent ± SD.

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