Extended Data Fig. 6: Purification of SNAP-tagged GAF-FL and GAF-ΔPOZ.
From: GAGA zinc finger transcription factor searches chromatin by 1D–3D facilitated diffusion
a, GAF-FL purification workflow. b, SDS-PAGE gel of GAF-FL elution after 6XHis pulldown, stained with Coomassie Blue. c, SDS-PAGE gel of GAF-FL elution after MBP pulldown, stained with Coomassie Blue. d, SDS-PAGE gel of AF546-GAF-ΔPOZ, scanned for AlexaFluor 546 fluorescence, then stained with Coomassie Blue. e, EMSA for GAF-ΔPOZ binding to Cy5-labeled hsp70 NCP DNA. GAF-ΔPOZ concentrations were 0, 4, 8, 12, 16, and 20 nM from left to right. DNA concentration was 3 nM. SDS-PAGE and EMSA gels shown in b-e were independently repeated once with similar results.
