Extended Data Fig. 4: Cryo-EM reconstruction of vRNAP assembly intermediates I3 and I4.
From: tRNA as an assembly chaperone for a macromolecular transcription-processing complex
(a) Selected 2D classes. (b) classification and refinement scheme. Domains are colored according to the scheme used in Fig. 2. (c) Final reconstruction for intermediate I3. (d) FSC plots for final reconstruction of intermediate I3. (e) Orientation distribution plot for final reconstruction of intermediate I3. (f) Posterior precision distribution plot for final reconstruction of intermediate I3. (g) Final reconstruction for intermediate I4. (h) FSC plots for final reconstruction of intermediate I4. (i) Orientation distribution plot for final reconstruction of intermediate I4. (j) Posterior precision distribution plot for final reconstruction of intermediate I4.
