Extended Data Fig. 8: Validation and additional analyses of d11 and i12 recombined clones.
From: Interactions between the genome and the nuclear lamina are multivalent and cooperative
(a) Total CAST and 129S1 allele-specific sequencing read counts per 25 kb from pA-DamID experiments, at the recombination sites and flanking regions, for clones used for deletion d11. Vertical dashed lines indicate the expected deletion on the CAST allele. (b) Domainogram and pA-DamD tracks for the 129S1 allele of non-recombined (“Control”, blue) and recombined (“d11”, red) cells from recombination d11. Data are plotted using WT chromosomal coordinates. Domainogram colour-key is displayed in (c). (c) Same plot as in Fig. 2c, right, for a wider window. (d) Screening of clones from recombination i12 by PCR. Clones were selected if PCR1 showed no amplification of the WT SB product and rec1 and rec2 products only were amplified from PCR2 and PCR3 respectively. For more details, see Extended Data Fig. 2A. (e-g) Domainograms (top) and pA-DamID tracks (bottom) comparing the 129S1 allele of non-recombined (“Control”, blue) and respective recombinations (“i12” (e), “i8” (f), “i9” (g), red). Data are plotted using WT chromosomal coordinates. Domainogram colour-key is displayed in (c).
