Fig. 3: NL-tethering subregions in LAD1 are autonomous.
From: Interactions between the genome and the nuclear lamina are multivalent and cooperative
a, CAST allele-specific pA-DamID track of NL interactions in WT cells, together with the position of one loxP integration within LAD1 that was used in combination with the anchor loxP to generate recombination i7. b, Effect of splitting LAD1 into two halves. Top: cartoon of a pA-DamID track of NL interactions of the CAST allele to illustrate the i7 inversion. The domainogram (middle) and pA-DamID tracks (bottom) of CAST allele NL interactions are shown, comparing nonrecombined and recombined cells from recombination i7. Data are plotted using the chromosomal coordinates of the inverted genome. The domainogram color key is displayed in d. Star, LAD1.1, which shows reduced NL contacts upon recombination; triangle, LAD1.2, whose NL interactions are largely unaffected by the recombination; arrowheads, iLAD regions that gain NL interactions upon recombination. c,d, Effects of isolating R4-5 (left) and R2-3 (right) in an iLAD. Rearrangements were generated by sequential inversion and deletion (Extended Data Fig. 5a,b). c, Cartoon of pA-DamID tracks of NL interactions of the CAST allele to illustrate the rearrangements. d, Domainogram (top) and pA-DamID tracks (middle) of CAST allele of cells with hopped but not recombined loxP (control, blue) and rearranged cells (experimental, red) in which R4-5 and R2-3 regions were isolated within an iLAD. pA-DamID tracks and associated domainograms are plotted using chromosomal coordinates of the rearranged genome. Arrows, NL interactions of R4-5 and R2-3 upon their isolation; arrowheads, R4-5 neighboring sequences gaining NL interactions. Bottom: schematics of NL interaction changes upon R4-5 and R2-3 isolation in an iLAD. A legend is provided in Fig. 2e. e,f, Same as c,d but after insertion of R4-5 and R2-3 close to LAD4.
