Fig. 4: R4-5 is the minimal sequence required for autonomous NL interactions.
From: Interactions between the genome and the nuclear lamina are multivalent and cooperative
a, Schematics of R4-5 truncations. Either one quarter (t1 and t2), one half (t2 and t3) or three quarters (t5 and t6) were truncated. b, R4-5 truncation results in a loss of NL interactions of remaining R4-5 and flanking iLAD sequences. Top: schematic of the Cas9-mediated truncation of R4-5. Truncations were performed after R4-5 isolation in iLAD sequences (Fig. 3c,d). Bottom: tracks showing the change in NL interactions in the R4-5 truncated clones compared to the nontruncated control (R4-5 isolated in iLAD sequences) (truncated minus control). Data were smoothed. Loosely dashed vertical lines mark the position of R4-5; densely dashed vertical lines mark the site of Cas9 editing within R4-5. Arrowheads indicate iLAD sequences losing NL interactions upon R4-5 truncation. c, No detectable effect of deletion of R4-5 from LAD1. Top: schematic of the Cas9-mediated deletion of R4-5. The domainogram (middle) and pA-DamID tracks (bottom) of CAST allele NL interactions are shown, comparing WT (control, blue) and Cas9-edited cells in which R4-5 was deleted (red).
