Extended Data Fig. 5: m6A landscape and gene expression analysis for lncRNAs Malat1, Neat1, and Kcnq1ot1.
From: m6A and the NEXT complex direct Xist RNA turnover and X-inactivation dynamics
a-c, m6A profile on lncRNAs Kcnq1ot1 (a), Malat1 (b), and Neat1 (c). Data are from ref. 21. MeRIP-seq data is shown in blue and input data in grey. For each panel, gene structure and transcriptional direction are shown below. Black boxes highlight METTL3 dependent m6A modification peaks. d-f, Expression levels for lncRNAs Malat1 (d), Neat1 (e), and Kcnq1ot1 (f) from ChrRNA-seq analysis. Cell lines are indicated at the top and clone names are indicated at the bottom. Samples are from FKBP12F36V-METTL3, METTL3-FKBP12F36V and transgene complementation experiments. Control samples are shown in grey, whereas the dTAG-13 treated (26 h) samples are shown in black. g-i, Expression levels for lncRNAs Malat1 (g), Neat1 (h), and Kcnq1ot1 (i) from ChrRNA-seq analysis. Cell lines are indicated at the top and clone names are indicated at the bottom. Samples are from YTHDC1-FKBP12F36V (left), ZCCHC8-FKBP12F36V and ZFC3H1-FKBP12F36V (right). Control samples are shown in grey, whereas the dTAG-13 treated (26 h) samples are shown in black. Note that two replicates are shown for each YTHDC1 clone.
