Extended Data Fig. 8: FKBP12F36V degron for nuclear m6A reader protein YTHDC1.
From: m6A and the NEXT complex direct Xist RNA turnover and X-inactivation dynamics
a,b, Profile of Tor1aip2 short and long isoforms in ChrRNA-seq samples of (a) METTL3-FKBP12F36V cells and (b) YTHDC1-FKBP12F36V degron cells (b). The arrows indicate the long isoform strongly upregulated in all of the dTAG-13 treated samples compared to untreated samples. Tor1aip2 gene structure is shown below. c, Boxplot showing the allelic ratio of X-linked genes (n = 263) from ChrRNA-seq analysis for YTHDC1 dTAG degron samples. The experimental design is as described in Fig. 1c. The red dashed line indicates allelic ratio at 0.5. Samples (two independent clones, Y9F and Y12H) and conditions are indicated above and below respectively. P-values, as indicated, are calculated using a two-sided paired t-test. Centre lines indicate the median, box limits indicate the first and third quartiles, and whiskers indicate 1.5× IQR. d, Barplot showing the expression level of Xist from ChrRNA-seq analysis for samples and conditions described in (c). Each bar represents one biological replicate.
