Fig. 3: Acute depletion of METTL3 stabilizes Xist RNA.
From: m6A and the NEXT complex direct Xist RNA turnover and X-inactivation dynamics
a, Schematic detailing RNA-SPLIT to assess the rate of Xist RNA turnover. b, Representative 3D-SIM images illustrating z-project of presynthesized (cyan) and newly synthesized (red) Xist molecules during expansion phase for WT (top; data from a previous study26) and METTL3 dTAG cells (bottom). Images are representative of 60, 100 and 140 min of pulse 2 labeling. Scale bar, 2 μm. c, Plot showing quantification of Xist RNA turnover during expansion (pink and light blue) and steady state (magenta and turquoise) for WT (pink and magenta; data from a previous study26), METTL3 dTAG (light blue and turquoise), with n > 20 cells per time point. Error bars represent the s.e.m. and the centers indicate the mean. d, As in b but images are representative of pulse 2 labeling at indicated times. Scale bar, 2 μm. e, Box plot showing Xist RNA transcription over time during expansion phase for WT (pink; data from a previous study26) and dTAG-13-treated METTL3 degron cells (blue), with n > 22 cells per time point. Significance was determined using a two-tailed Mann–Whitney test (**P = 0.0091, ***P = 0.008 and ****P < 0.0001). Center lines indicate the median, box limits indicate the first and third quartiles and whiskers indicate 1.5× the IQR.
