Extended Data Fig. 1: MeRIP confirms that m6A on Xist is dependent on METTL3 and acute depletion of METTL3 upregulates Xist and accelerates X-linked gene silencing.
From: m6A and the NEXT complex direct Xist RNA turnover and X-inactivation dynamics
a, Schematic illustrates the setup for the MeRIP experiment. b, Beanplot showing the m6A intensity distributions for previously defined m6A peaks. Orange and yellow back-to-back plots represent control (Ctrl) and dTAG conditions, respectively. The black solid lines denote the mean of each distribution. c, Genome Bowser view of MeRIP (blue) and input (black) signal across the Xist locus. The scale is indicated on the left, and Xist gene structure is shown below. The m6A peaks/clusters downstream of Xist A-repeat and downstream of Xist E-repeat are highlighted by dashed grey boxes. d-e, Barplots depicting m6A intensity for the representative peak located downstream of Xist A-repeat (d) and downstream of Xist E-repeat (e). Sample names and conditions are indicated below, and y-axis represents m6A intensity, that is log2 transformed value. The first four samples are from our previous study (GSE154707) and last two samples are generated from this study. Each bar represents one sample. f, Top: boxplots showing the calculated allelic ratio from input samples that were used in MeRIP experiments, with the number of gene for this analysis indicated above. Centre lines indicate the median, box limits indicate the first and third quartiles, and whiskers indicate 1.5× IQR. P values were calculated by a two-sided paired t-test. Bottom, barplots showing the Xist RNA expression (RPM) corresponding to the top panel. Sample order is as in d. Each bar represents one sample.
