Fig. 5: NPC binding requires a lack of FG-repulsive residues on the capsid surface.
From: Nuclear pore passage of the HIV capsid is driven by its unusual surface amino acid composition
a, Targeting of conical CLPs (noncovalently filled with sinGFP4) to HeLa cell NPCs was as in Fig. 1e. Indicated mutants were tested with identical scan settings as for wild-type capsids. b, Quantification of GFP signals on NPCs. Numbers are means; bars indicate the mean ± s.d. (n = number of quantified nuclei). Statistical significance between each mutant and the wild type was determined using an unpaired Student’s t-test: ****P < 0.001. Tabular data, including P values for statistical significance of group differences, are provided in Supplementary Data 1. Scale bar, 10 μm. Experiments were independently replicated three times with consistent outcomes.
