Extended Data Fig. 7: Quality controls for reporter screens.
From: Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus
(a) Reporter GFP signal during the reporter screens. The gray shade corresponds to non-fluorescent control cells. (b) Quantification of the data depicted in (a) as the mean fluorescence intensity. The black lines denote the geometric mean across three replicates. (c) SgRNA distribution width of the normalized counts in the different populations of the reporter screens, including all targeting guides. The score is given by the subtraction of the log2-transformed values for the 90th and 10th percentiles. (d) As in (a), but including only non-targeting controls. (e) Correlation of the normalized counts between all replicate samples per reporter screen. The populations are indicated by colored boxes. The axes are ordered by hierarchical clustering.
