Fig. 2: OCT4 acts as an Xist activator during differentiation.
From: Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus
a, Experimental setup used in b–d. Knockdown of Oct4 was induced 2 days before differentiation through dTAG removal. bFGF, basic FGF; ActA, activin A. b–d, Effect of Oct4 knockdown on Xist and Oct4 expression, assessed by reverse transcription (RT)–qPCR (b) and RNA-FISH (c,d). A total of 100 cells were counted manually per condition in c and representative pictures are shown in d. The experiments were performed in three biological replicates. P values of an unpaired two-sided t-test are indicated in the plot. e, Top: published ChIP-seq data, depicting binding of OCT4 at the Xist locus before and during differentiation in XY mES cells30. Bottom: RE track shows all Xist-controlling REs in the region12.
