Fig. 5: Sea2 is essential for the function of the SEAC.
From: Structure and function of the yeast amino acid-sensing SEAC–EGOC supercomplex
a, Immunoblots of phosphorylated and total Sch9 after starvation and repletion of amino acids (AA) in WT cells. b, Immunoblots of phosphorylated and total Sch9 after starvation and repletion of amino acids in Δsea4 cells. c, Immunoblots of phosphorylated and total Sch9 after starvation and repletion of amino acids in Δsea3 cells. d, Immunoblots of phosphorylated and total Sch9 after starvation and repletion of amino acids in Δsea2 cells. These cells have a lower overall TORC1 activity and slow growth. e, Quantification of relative Sch9 phosphorylation over 30 min of starvation and subsequent amino acid repletion in WT and SEACAT deletion strains. Data from three independent experiments are presented as mean values ± s.d. f, Quantification of relative Sch9 phosphorylation over 30 min of starvation and subsequent amino acid repletion in WT, Δsea2, sea2ΔN-ter and SEACCD cells. Data from three independent experiments are presented as mean values ± s.d. g, Immunoblot of phosphorylated and total Sch9 after starvation and repletion of amino acids in the sea2ΔN-ter strain.
