Extended Data Fig. 2: The role of INTS12 in transcriptional regulation and Integrator complex formation.
From: Integrator subunit INTS12 links ribotoxic stress to transcription-coupled nucleotide excision repair
a, The levels of INTS1 in control, INTS12 KO, and INTS12 KO cells reconstituted with stably expressed WT or 3 A INTS12-Flag were analyzed by WB. b, Lysates from control or INTS12 KO cells that were treated with CHX for the indicated time were analyzed by WB. c, d, Gradient centrifugation using nuclear extracts from control or INTS12 KO cells (c) or INTS12 KO cells reconstituted with stably expressed WT or 3 A INTS12 (d). The fractionated samples were analyzed using SDS-PAGE followed by WB. e, Schematic illustrating the preDRB-nRNA-seq. Cells released from DRB pretreatment were labeled with 4-SU at the indicated times. f, Heatmaps of preDRB-nRNA-seq data from the TSS into the first 60 kb of active genes larger than 60 kb in control and INTS12 KO cells, followed by the indicated times. g, Metaplots showing the average level of nascent transcription of genes defined in f in control or INTS12 KO cells. Wavefront positions are defined as the distance from the TSS where the reads density drops below an arbitrary threshold (dashed line). All Western blots are representative of three independent experiments.
