Table 3 Validation of the assembly quality and improved gene annotation of tea plant genome using three methodologies.
Validation of assembly quality | Number | Percentage (%) |
|---|---|---|
BUSCO validation | ||
Total BUSCO groups | 1,440 | 100 |
Complete single-copy BUSCOs | 1,180 | 81.9 |
Complete duplicated BUSCOs | 151 | 10.5 |
Fragmented BUSCOs | 44 | 3.1 |
Missing BUSCOs | 65 | 4.5 |
BAC validation | ||
Total BACs (#) | 18 | 100 |
Total length (bp) | 2,080,846 | 100 |
Aligned BACs (bp) | 1,182,063 | 98.30 |
PCR validation | ||
Total PCR experiments | 24 | 100 |
Success PCR experiments | 22 | 91.67 |
Validation of gene prediction | ||
Total BUSCO groups | 1,440 | 100 |
Complete single-copy BUSCOs | 1,068 | 74.2 |
Complete duplicated BUSCOs | 173 | 12.0 |
Fragmented BUSCOs | 118 | 8.2 |
Missing BUSCOs | 81 | 5.6 |
