Fig. 5

PCL compound toxicity in uninfected cells. Of the 1,278 PCL compounds tested, 126 PCL compounds are found to be toxic, as shown in red, and listed in PCL compounds excluded due to toxicity in uninfected cells²⁹. A549 cells were treated with PCL compounds in duplicates according to the screening wet-lab protocol, however, in absence of HAdV infection for 3.5 days. Doxorubicin hydrochloride (Prestw-438) was used as a positive control for cytotoxicity, at a final concentration of 10 µM, and the corresponding concentration of the drug solvent DMSO was used as a negative control. Cell viability was determined by PrestoBlue assay. PrestoBlue fluorescence intensities of each well were normalized per plate to negative control values at 0 and positive controls at 1. Compounds were considered toxic, when the normalized value for all replicates was higher than the average +3σ (standard deviation, SD) of the DMSO negative control for the corresponding plate. X-axis indicates compounds by their PCL identifier (PCL ID, see PCL compounds tested in the screening procedure²⁹). Normalized average PrestoBlue read-outs are depicted on the y-axis.