Fig. 4

Immunohistochemistry analysis of top ranked cardiac-enriched candidates. Immunohistochemistry analysis of the top ranked candidates in each subcellular classification (Mitochondrion, plasma membrane, other organelles (ER, golgi apparatus, peroxisomes, lysosomes), cytosol, and the secretory pathway in healthy human ventricular tissues from the Human Protein Atlas. Immunohistochemical stain intensity was assessed independently as either weak, moderate or strong and reported as protein expression score (PES). COX20, moderate PES; FAM162A, weak PES; Tmem65, moderate PES; MCT1, moderate PES; SLC12A7, moderate PES; TLN2, moderate PES; MSN, moderate PES; FLOT1, moderate PES; SNTA1, moderate PES; DYSF, moderate PES; AOC3, negative PES; REEP5, moderate PES; STOM, weak PES; CTNND1, moderate PES; LAMA2, strong PES; LAMB2, strong PES; BGN, moderate PES; MYH7, strong PES; TNNT2, strong PES; TNNI3, strong PES. Scale bar, 25μm. All images shown are representative of approximately 5 distinct regions of interest (ROIs) assessed per sample, n = 3 independent patient tissue blocks.