Fig. 5
Immunblot analysis of protein expression across multiple tissues and microsomal fractions. Immunoblot analysis of (a) FAM162A, (b) MCT1, and (c) COX20 protein expression levels in mouse tissues. Blots were incubated with either anti-DDK antibody or FAM162A, MCT1 or COX20. Immunodepletion experiments were carried out by incubating primary antibodies together with HEK overexpression lysates. Coomassie blue gels were performed to visualize protein loading across all samples. (d) Immunoblot analysis of FAM162A, MCT1, and COX20 in isolated adult cardiac cytosolic fraction and enriched membrane microsomes from 10 mouse hearts. Blots were probed with primary antibodies as indicated, and in parallel, samples were run on a Coomassie blue gel. All immunoblots shown are representative of 3 total immunoblots performed, n = 3 independent biological replicates. Original uncropped immunoblots are provided in Supplemental Fig. 9. All original uncropped images were uploaded to figshare (https://doi.org/10.6084/m9.figshare.11844972.v12).
