Online-only Table 1 Diagnostics tests which have been carried out on the IDEAL samples to screen the samples for pathogens and to produce haematological profiles.
From: IDEAL, the Infectious Diseases of East African Livestock project open access database and biobank
Sample Type | Test | Test result |
|---|---|---|
Faecal | Direct Baermann | Dictyocaulus viviparus (L1) |
McMaster | Coccidia spp., Long tailed worms: Chabertia spp., Oesophagostomum spp., Bunostomum spp., Medium tailed worms: Cooperia spp., Haemonchus spp., Moniezia spp., Nematodirus spp., Strongyle eggs, Strongyloides spp., Toxocara spp., Trichuris spp. | |
Flotation | Coccidia spp., Nematodirus spp., Strongyle eggs, Strongyloides spp., Toxocara spp. | |
Sedimentation | Coccidia spp., Fasciola spp., Strongyle eggs, Toxocara spp. | |
Sporulation | Eimeria alabamensis, Eimeria auburnensis, Eimeria bovis, Eimeria cylindrica, Eimeria subspherica, Eimeria zuernii | |
Guildhays Test | Rotavirus | |
Faecal culture and larvae identification | Long tailed worms: Chabertia spp., Oesophagostomum spp. and Bunostomum spp., Medium tailed worms: Cooperia spp., Haemonchus spp., Nematodirus spp., Short tailed worms: Trichostrongylus spp., Teladorsagia (Ostertagia) spp. | |
Peripheral ear vein | Giemsa stain | Anaplasma spp., Babesia spp., Ehrlichia ruminatum, Theileria spp., Trypanosoma spp. |
Blood smears stained with Diff Quick (Kyron, South Africa) | Absolute eosinophil count, Absolute lymphocyte count, Absolute monocyte count, Absolute neutrophil count | |
Dark ground microscopy | Microfilaria spp., Trypanosoma spp. | |
EDTA tube | pocH-100iV Diff (Sysmex® Europe GmbH) | Absolute eosinophil count, Absolute lymphocyte count, Haematocrit, Hemoglobin concentration, Mean cell haemoglobin, Mean corpuscular hemoglobin concentration, Mean platelet volume, Platelet count, Platelet distribution width, Platelet larger cell ratio, Red cell count, Red cell distribution width - coefficient of variation, Red cell distribution width - standard deviation, Total white cell count |
Hawksley micro-hematocrit reader | Packed cell volume | |
Reverse Line Blot (RLB) | Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Babesia bicornis, Babesia bigemina, Babesia bovis, Babesia caballi, Babesia canis, Babesia catch-all 1, Babesia catch-all 2, Babesia divergens, Babesia felis, Babesia gibsoni, Babesia gibsoni Japan, Babesia gibsoni USA, Babesia leo, Babesia major, Babesia microti, Babesia occultans, Babesia rossi, Babesia sp. (sable), Babesia venatorum, Babesia vogeli, Ehrlichia / Anaplasma catch-all, Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia ruminatum, Ehrlichia sp. (Omatjenne), Theileria / Babesia catch-all, Theileria annae, Theileria annulata, Theileria bicornis, Theileria buffeli, Theileria catch-all, Theileria equi, Theileria lestoquardi, Theileria mutans, Theileria ovis, Theileria parva, Theileria separata, Theileria sp. (buffalo), Theileria sp. (kudu), Theileria sp. (sable), Theileria taurotragi, Theileria velifera | |
PCR | Bluetongue Virus, Capripox, Ehrlichia ruminatum, Epizootic hemorrhagic disease, Trypanosoma brucei, Trypanosoma congolense, Trypanosoma vivax, p104 for Theileria parva | |
Ziehl Neelsen staining for microscopy | Cryptosporidium spp. | |
Plain tube | Refractometer (model RHC-200ATC, Westover Scientific) | Total serum proteins |
ELISA | Anaplasma marginale, Babesia bigemina, Bluetongue Virus, Bovine Leukaemia Virus, Bovine Respiratory Syncytial Virus, Bovine Virus Diarrhoea, Bovine Virus Diarrhoea (antigen), Epizootic Haemorrhagic Disease Virus, Infectious Bovine Rhinotracheitis, Leptospira hardjo, Neospora Caninum, Parainfluenza 3 Virus, Theileria mutans, Theileria parva, ILG-10, TgF | |
Ecotoparasites | Identification of ticks | Amblyomma variegatum, Riphicephalus appendiculatus |
Bacteriology | Bacteriology conclusion | Actinomyces sp., Actinomycetes, Arcanobacterium spp., Bacillus sp., Clostridium spp., Corynebacterium spp., Dermatophilus congolensis, E. coli, Gram negative bacilli, Gram positive bacilli, Gram positive cocci, Klebsiella ozaenae, Klebsiella pneumoniae, Listeria spp., Micrococcus spp., Non-pathogenic Staphylococci, Pasteurella multocida, Salmonella spp., Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus spp., Streptococcus spp., Thin rods (Bacteria), Unidentified colony, Weksella zoohelcum |
