Online-only Table 2 The samples collected from the IDEAL cattle and their uses.
From: IDEAL, the Infectious Diseases of East African Livestock project open access database and biobank
Sample Label | Sample Description | Uses | Animal Sampled | Sample Type |
---|---|---|---|---|
BCI | Bacterial Isolates from Swabs | Diagnostics | Calves | Clinical |
BFI | Bacterial Isolates from Faeces | Diagnostics | Calves | Clinical |
BGA | Aliquot of heparinised blood in 1.5 ml eppendorf tube | Diagnostics | Calves | Routine |
BGB | Aliquot of heparinised blood in 1.5 ml eppendorf tube | Diagnostics | Calves | Routine |
BGC | Aliquot of heparinised blood in 1.5 ml eppendorf tube | Calves | Routine | |
BKS | Jugular blood thick smear. Several drops of blood from 5 ml EDTA plastic tubes was used to generate two thick smears of non-peripheral (jugular) blood. A separate slide is used for each smear and they where stored without further processing (unfixed & unstained) | Diagnostics | Calves & dams | Routine |
BMI | Bacterial Isolates from milk from California Mastitis Test (CMT) positive quarters | Diagnostics | Dam | Clinical |
BNS | Jugular blood thin smear. Several drops of blood from 5 ml EDTA plastic tubes was used to generate two thin smears of non-peripheral (jugular) blood. A separate slide is used for each smear and they were fixed with absolute methanol for 5 minutes before storing | Diagnostics | Calves & dams | Routine |
CMS | ‘Clinical Miscellaneous Samples’; a heterogeneous group of samples whose collection is ‘in addition’ to the compulsory routine samples | Clinical diagnosis | Calves | Clinical |
HSV | 1.8 ml plastic cryotubes of jugular blood decanted from 10 ml heparinised glass tubes | Diagnostics | Calves | Clinical |
MBE | Mix of jugular blood and magic buffer (5 ml: 5 ml) in 10 ml EDTA glass tubes | Host genetics | Calves & dams | Routine |
MKS | Thick smear from peripheral blood collected from the marginal ear vein | Screening of blood borne protozoa | Calves | Routine |
MLK | 40–50 ml plastic tubes with white lead containing 40–50 ml of milk pulled from all healthy quarters (i.e. quarters presenting with no macroscopic signs of mastitis) | Nutrition studies | Dams | Routine |
MNS | Thin smear from peripheral blood collected from the marginal ear vein | Manual haematology & screening of blood borne protozoa | Calves | Routine |
NMB | Jugular blood thin smear for new methylene blue staining and reticulocite count | Diagnostics | Calves | Clinical |
RED | Jugular blood in 5 ml EDTA plastic tubes | Automated haematology, manual PCV, screening of trypanosomes, PCR | Calves & dams | Routine |
RLE | Mix of EDTA jugular blood and RNALater (500 µl: 1.3 ml) in 1.8 ml plastic cryotubes with external thread | RNA studies | Calves | Routine |
SER | 1.8 ml plastic cryotubes containing serum derived from centrifugation of jugular blood in 10 ml glass-made plain vacutainers | Serology screening | Calves & dams | Routine & clinical |