Fig. 2

Plots showing quality of libraries after filtering to Swissprot protein entries. (a) Histogram of mouse protein abundance from paxDB database with proteins identified by proteotypic peptides in the final library overlayed. The library covers almost all of the most abundant proteins in the mouse proteome. (b) The numbers of proteins identified by 1–4 and 5 or more proteotypic peptides. (c) The difference in iRT score between peptides identified in both cell culture samples and re-analysed tissue samples. (d) Distribution of similarity scores (cosine of matched signal intensity vectors) between cell culture and tissue derived data. (e) Venn diagram for numbers of proteins uniquely identified across all combination of sample sets.